Pasteurized Substrate Production Masterclass
Pasteurized Substrate for Oyster Mushrooms and Shiitake: Take your skills to the next level
Master the art of bulk pasteurization: A unique 5-day learning experience in Greece, perfect for experienced growers and driven newcomers.
We invite you to take part in an immersive program focused on the production of pasteurized substrate for Oyster Mushrooms, King Oyster and Shiitake. Designed for both beginners and experienced professionals, this in-depth advisory program provides the knowledge and hands-on expertise needed to refine your process and improve efficiency. Whether you’re looking to streamline production or solve persistent challenges, this course will provide practical solutions and insights to optimize your pasteurization process.
All of this unfolds within Manitaria, a full-scale production facility specializing in tunnel pasteurization and Pleurotus substrate production, giving you direct access to real-world expertise and operations.
Join us to refine your skills, enhance process control, and discover new ways to improve efficiency in pasteurized substrate production.

Ready to thrive together?
Join our Pasteurization Substrate Production Journey and learn from experts who live and breathe real-world solutions.
May 2025
2 499 (excl. VAT)
Greece
The masterclass
A 5-Day program built on decades of Pasteurization Expertise.
This guidance program follows the same steps as our Substrate and Wood Fungi Production Course, but with a specialized focus on pasteurization. The topics are tailored to cover pasteurization in greater depth, offering more hands-on practice and advanced insights. We provide a truly comprehensive learning experience – no shortcuts, just the real deal.
Details
- The company Κεχαγιά (Manitaria) in Greece is our unique learning environment
- Nearest airport: Thessaloniki International Airport (SKG), Greece
- Training language: English
- The focus is on pasteurized substrate production specifically for Oyster Mushrooms, but also King Oyster and Shiitake
- Better quality with smaller groups: we opt for a maximum of 16 participants (minimum 12)
- For beginners and professionals refining their expertise, as well as past participants of the Substrate and Wood Fungi Production Course seeking deeper, non-overlapping insights
- 2 499 (excl. VAT)
The program
This course is highly practical and hands-on. Since you will be following the production process on-site, we maintain a certain level of flexibility in the daily program. The course is reality-based and will be adapted to the actual production flow of Manitaria’s facility. Each course day will be structured with a balance of theoretical sessions, hands-on practice, site visits, and process checks.
In general, you will go through all the steps of the production process throughout the week. You will gain a deep understanding of both the theory and its real-world application. During the sessions, there is a lot of time for your personal questions.
Raw Materials & alternatives
Cutting & Watering
Fermentation:
- Preparation of the hip
- Filling of the bunker
- Turning ( none – once or twice)
Pasteurization:
- Filling the tunnel of pasteurization
- Differences between a bunker of fermentation and a tunnel of pasteurization
Spawning & packing (blocks):
- What is a spawning hall
- What is spawn
- Packing line
- Cleaning & hygiene
Incubation
Visit of a fructification rooms
Feedback and open discussion at the end of the training
End of course at +/- 5 p.m

The company Κεχαγιά (Manitaria) in Greece: A Unique hands-on learning environment
This isn’t just a course – it’s a rare opportunity to learn inside the company itself, where you’ll see real production in action, gain hands-on insights from industry experts, and understand the workflow from up close. Participants will not just observe but actively apply every production step in a real facility.
Or watch the company’s video here:
Raw materials to harvest: Learn from the best
This hands-on course, guided and developed by our MyAc expert Christelle Chevalier and Orazio Tietto, General Manager of Azienda Agricola, blends theory and practice to cover every stage of the process – from selecting raw materials to incubation. Participants will also visit an oyster mushroom farm to see firsthand how oyster mushrooms are produced in real-world conditions.
Register here!

Learning the production techniques on-site
Special Highlights
- Mushroom Farm Visit: As part of the journey, we’ll visit a mushroom farm where Oyster mushrooms are cultivated. This visit will provide valuable insights into how pasteurized substrate is used in a commercial setting.
- Practical Focus: This expert-led program goes even further in hands-on learning than our valuable on-site courses, with a significant portion dedicated to practical learning, ensuring participants leave with the confidence and skills to implement what they’ve learned. You have the opportunity to use the tunnel, you see the incubation, you gain insight into the duration of each proces and more.
Whether you’re an aspiring mushroom grower or a seasoned cultivator looking to refine your techniques, this course offers the perfect blend of theoretical knowledge and practical application to help you succeed.
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We will open more course editions to our on-site courses on Mycelium Production in 2025.
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Our next editions
Pasteurization Production Course (May 2025)
5 May 2025 – 9 May 2025
Only 6 of 16 spots available

What You'll Dive Into:
- Raw Materials: Learn how to select and prepare the best raw materials for optimal substrate quality.
- Fermentation: Understand the role of fermentation in substrate preparation and how to manage this phase effectively.
- Pasteurization: Gain practical and theoretical knowledge on pasteurization techniques to create a substrate free from contaminants.
- Spawning: Learn the process of integrating mushroom spawn into the substrate for successful colonization.
Some more topics?
- Block Formation: Master the techniques for forming substrate blocks that promote healthy mycelium growth.
- Incubation: Understand the incubation phase, including environmental controls and monitoring for optimal results.
- Hygiene: Discover best practices for maintaining hygiene throughout the production process to ensure high-quality substrate.
- Only 16 spots available – don’t miss out on this exclusive opportunity! Add yourself to the waiting list today.
The in-depth guidance is thoughtfully designed to combine:

Theory
Gain an in-depth understanding of the scientific principles behind each stage of substrate production. Topics will include substrate composition, pasteurization techniques, conditions for optimal mycelium colonization and the microbiological factors that influence production success.

Practice
Participate in hands-on workshops to experience the entire production process firsthand. You’ll have the opportunity to engage directly with the tools, materials, and techniques used in professional pasteurized substrate production, manage the entire process from start to finish.
Experts of this edition

Christelle Chevalier

Orazio Tietto
Extra information
The company Manitaria is your unique learning environment.
This Google Maps link will guide you to the course location.
The production unit is located in Kilkis 61100, with the growing rooms situated 12 km from the city in the small village of Pedino. The compost yard, where the training will take place, is 1.5 km from the center of Pedino.
We recommend two hotels in Kirkis, within 15 km of the course location:
The Habitat Hotel and Kristonia Hotel.
Both hotels are surrounded by cozy bars and restaurants, offering plenty of opportunities to connect with fellow participants in the evenings. Whether you prefer gathering in a nearby horeca location or the comfort of your hotel lobby, the choice is yours!
While we recommend the two selected hotels for their convenience and proximity, booking one of them is not mandatory. The town offers plenty of other accommodation options to suit your preferences and budget.
Your course fee includes:
- Daily lunch at a traditional Greek restaurant, offering a light yet satisfying meal.
- Unlimited refreshments during breaks, including coffee, tea, snacks, fresh fruit, water, and juices.
- A special welcome dinner on the first evening to meet fellow participants and instructors.
Please note: All other dinners are at your own expense, allowing you the flexibility to explore local dining options.
For those who do not have private transportation (via rental car or personal vehicle), we offer an optional shuttle service for added convenience.
Since the recommended hotels are located in Kilkis, about 15 km from the course location, this shuttle service will transport participants between the two designated hotels in Kilkis and the Mycelia Academy course venue.
The shuttle operates twice daily, in the morning and evening, ensuring you arrive on time for the courses.
The service is available for €75 per person per course week.
The shuttle service fee will be billed directly by Mycelia Academy.
This service is entirely optional. If you prefer, you are welcome to arrange your own transportation to and from the course venue. For more details on transport options, please check the next bullet.
For Participants Staying in Other Hotels
Participants who choose accommodations other than the two recommended hotels are still welcome to use the shuttle. However, please note:
✔ Pick-up and drop-off will only take place at the two designated hotels in Kilkis.
✔ If you stay elsewhere, you will need to arrange your own transport to and from one of these hotels to use the shuttle service.
If you arrive by plane, the nearest airport is Thessaloniki International Airport (SKG).
To get from the airport to Kilkis (where the recommended hotels are located), there are several transport options available, which you can book directly with the providers. To assist you, here are some resources to explore different transportation possibilities:
- Transport options from Thessaloniki Airport (SKG) to Kilkis
- Transport options from nearby airports to Kilkis
If you choose to travel by public transport (bus, train, taxi), you can also make use of our optional shuttle service from the two selected hotels in Kilkis to the course location (see the previous bullet for details).
Would you prefer more flexibility during your stay? Renting a car is a great option. You’ll find many car rental services available. For reference, you can check out Hellas Chauffeur, where rental prices start at approximately €128 for five days. Of course, you are free to choose any other provider that suits your needs!